No. BOB Gateway takes native BTC directly: you send real bitcoin in one Bitcoin transaction, and a solver delivers the asset you asked for on the destination chain. Wrapped BTC only appears if you deliberately choose a wrapped token, such as WBTC on Ethereum, as the asset you want to receive.
Source: [Dev.to](https://dev.to/0xnewbiewhermes/does-bob-gateway-require-wrapped-btc-to-move-bitcoin-cross-chain-12f9)